Am. J. Respir. Cell Mol. Biol., Volume 19, Number 4, October, 1998 537-542

RAPID COMMUNICATION
Expression of Fas (CD95) and FasL (CD95L) in Human Airway Epithelium

Kimm J. Hamann,^* Delbert R. Dorscheid,^* Franklin D. Ko, Amber E. Conforti, Anne I. Sperling, Klaus F. Rabe, and Steven R. White

Section of Pulmonary and Critical Care Medicine, Division of Biological Sciences, University of Chicago, Chicago, Illinois; and Krankenhaus Großhansdorf, Großhansdorf, Germany

The cell surface molecule Fas (CD95) is a member of the tumor necrosis factor receptor family. Ligation of the Fas receptor can lead to induction of apoptosis in inflammatory cells. It has been suggested that expression of the Fas receptor and its ligand (FasL) in airway epithelium may modulate the inflammatory response commonly found in asthmatic lungs. We examined Fas and FasL expression on primary human tissues, on bronchial epithelial cells in primary culture, and on the immortalized human airway epithelial cell line, 1HAE_o. Receptor and ligand expression were demonstrated using multiple antibodies and multiple techniques, including immunohistochemistry, flow cytometry, Western blots, and reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical staining demonstrated that both columnar and basal cells of intact human lung tissues expressed cell surface Fas and FasL. In addition, both primary cultured and immortalized 1HAE_o cells expressed cell surface Fas and FasL, as demonstrated by flow cytometry; expression of Fas and FasL was confirmed at the transcription level using RT-PCR and, for additional confirmation of FasL, using Western blots. We demonstrate that both Fas and FasL are expressed by human airway epithelial cell subtypes. Expression of these molecules may play an important role in regulation of the inflammatory response.

This article has been cited by other articles:

				Y. A. Mebratu, B. F. Dickey, C. Evans, and Y. Tesfaigzi The BH3-only protein Bik/Blk/Nbk inhibits nuclear translocation of activated ERK1/2 to mediate IFN{gamma}-induced cell death J. Cell Biol., November 3, 2008; 183(3): 429 - 439. [Abstract] [Full Text] [PDF]

				R. A. Bem, A. W. Farnand, V. Wong, A. Koski, M. E. Rosenfeld, N. van Rooijen, C. W. Frevert, T. R. Martin, and G. Matute-Bello Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L314 - L325. [Abstract] [Full Text] [PDF]

				M. E. De Paepe, S. Gundavarapu, U. Tantravahi, J. R. Pepperell, S. A. Haley, F. I. Luks, and Q. Mao Fas-Ligand-Induced Apoptosis of Respiratory Epithelial Cells Causes Disruption of Postcanalicular Alveolar Development Am. J. Pathol., July 1, 2008; 173(1): 42 - 56. [Abstract] [Full Text] [PDF]

				G. Matute-Bello, M. M. Wurfel, J. S. Lee, D. R. Park, C. W. Frevert, D. K. Madtes, S. D. Shapiro, and T. R. Martin Essential Role of MMP-12 in Fas-Induced Lung Fibrosis Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 210 - 221. [Abstract] [Full Text] [PDF]

				D. R. Dorscheid, B. J. Patchell, O. Estrada, B. Marroquin, R. Tse, and S. R. White Effects of corticosteroid-induced apoptosis on airway epithelial wound closure in vitro Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L794 - L801. [Abstract] [Full Text] [PDF]

				F. Heinzelmann, V. Jendrossek, K. Lauber, K. Nowak, T. Eldh, R. Boras, R. Handrick, M. Henkel, C. Martin, S. Uhlig, et al. Irradiation-Induced Pneumonitis Mediated by the CD95/CD95-Ligand System. J Natl Cancer Inst, September 6, 2006; 98(17): 1248 - 1251. [Abstract] [Full Text] [PDF]

				Y. Tesfaigzi Roles of Apoptosis in Airway Epithelia Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 537 - 547. [Abstract] [Full Text] [PDF]

				P. Chanvorachote, U. Nimmannit, L. Wang, C. Stehlik, B. Lu, N. Azad, and Y. Rojanasakul Nitric Oxide Negatively Regulates Fas CD95-induced Apoptosis through Inhibition of Ubiquitin-Proteasome-mediated Degradation of FLICE Inhibitory Protein J. Biol. Chem., December 23, 2005; 280(51): 42044 - 42050. [Abstract] [Full Text] [PDF]

				T. R. Martin, N. Hagimoto, M. Nakamura, and G. Matute-Bello Apoptosis and Epithelial Injury in the Lungs Proceedings of the ATS, October 1, 2005; 2(3): 214 - 220. [Abstract] [Full Text] [PDF]

				C. D. Douillet, W. P. Robinson III, B. L. Zarzaur, E. R. Lazarowski, R. C. Boucher, and P. B. Rich Mechanical Ventilation Alters Airway Nucleotides and Purinoceptors in Lung and Extrapulmonary Organs Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 52 - 58. [Abstract] [Full Text] [PDF]

				S. Bao, Y. Wang, P. Sweeney, A. Chaudhuri, A. I. Doseff, C. B. Marsh, and D. L. Knoell Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L36 - L42. [Abstract] [Full Text] [PDF]

				N. S. Undevia, D. R. Dorscheid, B. A. Marroquin, W. L. Gugliotta, R. Tse, and S. R. White Smad and p38-MAPK signaling mediates apoptotic effects of transforming growth factor-{beta}1 in human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L515 - L524. [Abstract] [Full Text] [PDF]

				M. Nakamura, G. Matute-Bello, W. C. Liles, S. Hayashi, O. Kajikawa, S.-M. Lin, C. W. Frevert, and T. R. Martin Differential Response of Human Lung Epithelial Cells to Fas-Induced Apoptosis Am. J. Pathol., June 1, 2004; 164(6): 1949 - 1958. [Abstract] [Full Text] [PDF]

				R. Tse, B. A. Marroquin, D. R. Dorscheid, and S. R. White {beta}-Adrenergic agonists inhibit corticosteroid-induced apoptosis of airway epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L393 - L404. [Abstract] [Full Text] [PDF]

				K. H. Albertine, M. F. Soulier, Z. Wang, A. Ishizaka, S. Hashimoto, G. A. Zimmerman, M. A. Matthay, and L. B. Ware Fas and Fas Ligand Are Up-Regulated in Pulmonary Edema Fluid and Lung Tissue of Patients with Acute Lung Injury and the Acute Respiratory Distress Syndrome Am. J. Pathol., November 1, 2002; 161(5): 1783 - 1796. [Abstract] [Full Text] [PDF]

				Z. O-Quan Shi, M. J. Fischer, G. T. De Sanctis, M. R. Schuyler, and Y. Tesfaigzi IFN-{gamma}, But Not Fas, Mediates Reduction of Allergen-Induced Mucous Cell Metaplasia by Inducing Apoptosis J. Immunol., May 1, 2002; 168(9): 4764 - 4771. [Abstract] [Full Text] [PDF]

				D. R. DORSCHEID, K. R. WOJCIK, S. SUN, B. MARROQUIN, and S. R. WHITE Apoptosis of Airway Epithelial Cells Induced by Corticosteroids Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1939 - 1947. [Abstract] [Full Text] [PDF]

				G. Matute-Bello, W. C. Liles, C. W. Frevert, M. Nakamura, K. Ballman, C. Vathanaprida, P. A. Kiener, and T. R. Martin Recombinant human Fas ligand induces alveolar epithelial cell apoptosis and lung injury in rabbits Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L328 - L335. [Abstract] [Full Text] [PDF]

				S. R. White, P. Williams, K. R. Wojcik, S. Sun, P. S. Hiemstra, K. F. Rabe, and D. R. Dorscheid Initiation of Apoptosis by Actin Cytoskeletal Derangement in Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 282 - 294. [Abstract] [Full Text] [PDF]

				S. R. White, K. R. Wojcik, D. Gruenert, S. Sun, and D. R. Dorscheid Airway Epithelial Cell Wound Repair Mediated by {alpha}-Dystroglycan Am. J. Respir. Cell Mol. Biol., February 1, 2001; 24(2): 179 - 186. [Abstract] [Full Text]

				M. E. De Paepe, L. P. Rubin, C. Jude, A. M. Lesieur-Brooks, D. R. Mills, and F. I. Luks Fas ligand expression coincides with alveolar cell apoptosis in late-gestation fetal lung development Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L967 - L976. [Abstract] [Full Text] [PDF]

				K. J. Hamann, J. E. Vieira, A. J. Halayko, D. Dorscheid, S. R. White, S. M. Forsythe, B. Camoretti-Mercado, K. F. Rabe, and J. Solway Fas cross-linking induces apoptosis in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, March 1, 2000; 278(3): L618 - L624. [Abstract] [Full Text] [PDF]

				I. Durieu, C. Amsellem, C. Paulin, M.-T. Chambe, J. Bienvenu, G. Bellon, and Y Pacheco Fas and Fas ligand expression in cystic fibrosis airway epithelium Thorax, December 1, 1999; 54(12): 1093 - 1098. [Abstract] [Full Text]