Am. J. Respir. Cell Mol. Biol., Volume 19, Number 6, December, 1998 875-880

Structure of the Murine Secretory Leukoprotease Inhibitor (Slpi) Gene and Chromosomal Localization of the Human and Murine SLPI Genes

Toshiaki Kikuchi, Tatsuya Abe, Sachiko Hoshi, Nobumichi Matsubara, Yasuyuki Tominaga, Ken Satoh, and Toshihiro Nukiwa

Department of Respiratory Oncology and Molecular Medicine, Division of Cancer Control, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan

Secretory leukoprotease inhibitor (SLPI) is a serine protease inhibitor involved in antineutrophil elastase protection at inflammatory sites. To elucidate both the function and regulation of SLPI in vivo, we isolated and characterized the mouse Slpi gene. An entire 3-kb mouse Slpi gene fragment was sequenced, including an 0.8-kb 5'-flanking region, the 2.2-kb Slpi gene, and a 0.1-kb 3'-flanking region. The mouse Slpi gene spans 2,222 base pairs containing four exons and three introns. All splicing borders between exons and introns are conserved as predicted by GT-AG rules. Using primer extension analysis, the transcription start site was located 20 nucleotides upstream from the methionine (ATG) initiation codon. At the defined transcription start site, the sequence TCA₊₁GAGC is present. These results indicate that both mouse and human genomic structure are highly conserved. Using fluorescence in situ hybridization, we confirmed that, consistent with the genomic similarity, the human SLPI gene is localized on chromosome 20q12-13.2 and the mouse homologue on chromosome 2H, which are syntenic with each other.

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