help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemjabbar, H.
Right arrow Articles by Lafuma, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemjabbar, H.
Right arrow Articles by Lafuma, C.

Am. J. Respir. Cell Mol. Biol., Volume 20, Number 5, May, 1999 903-913

Overexpression of Alveolar Macrophage Gelatinase B (MMP-9) in Patients with Idiopathic Pulmonary Fibrosis
Effects of Steroid and Immunosuppressive Treatment

Hassan Lemjabbar, Philippe Gosset, Emmanuelle Lechapt-Zalcman, Marie-Laure Franco-Montoya, Benoit Wallaert, Alain Harf, and Chantal Lafuma

INSERM U296 de Physiologie Respiratoire, Faculté de Médecine, Créteil; INSERM U416 de Pathologie Immuno-Allergologique Respiratoire, Institut Pasteur; and Service de Pneumologie et Immuno-Allergologie, Hôpital André, Calmette, Lille, France

Alveolar macrophages (AM) express gelatinase B, a member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components. We evaluated the expression of gelatinase B in the course of idiopathic pulmonary fibrosis (IPF) by studying alveolar macrophages in culture AM and bronchoalveolar lavage fluid from 12 untreated patients with IPF, 11 patients with IPF under treatment with steroid and immunosuppressive agents, and 10 control subjects. By using zymography and quantitative image analysis, latent gelatinase B, as well an 88-kD active form, were investigated in culture medium (24 h) of AMs and were found to be significantly increased (P < 0.01) in untreated patients exhibiting severe IPF when compared with control subjects (4.1 ± 1.7 versus 0.3 ± 0.2 105 arbitrary units [AU]/104 AM for the 92-kD form). Concomitant studies of gelatinase B levels associated with cultured AM extracts or freshly harvested AM showed similar results, both at the mRNA and protein levels, respectively. Immunocytochemical studies on freshly harvested AM demonstrated that the enzyme was located mainly at the cell, suggesting some involvement of gelatinase B in AM migration. In contrast, gelatinase B activity secreted by AM tended to be normal in patients with IPF under steroid and immunosuppressive treatment. Simultaneously, level of the gelatinase B activity in epithelial lining fluid was increased in untreated IPF patients, whereas it was normal in treated patients. These results suggest that AM of patients with IPF are primed for gelatinase B expression and that steroid and immunosuppressive treatment induces negative modulation of the gelatinase B overexpression. We conclude that gelatinase B may play a role in lung remodeling in IPF.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
S. McKeown, A. G. Richter, C. O'Kane, D. F. McAuley, and D. R. Thickett
MMP expression and abnormal lung permeability are important determinants of outcome in IPF
Eur. Respir. J., January 1, 2009; 33(1): 77 - 84.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. W. Huh, D. S. Kim, Y.-M. Oh, T. S. Shim, C. M. Lim, S. D. Lee, Y. Koh, W. S. Kim, W. D. Kim, and K. R. Kim
Is Metalloproteinase-7 Specific for Idiopathic Pulmonary Fibrosis?
Chest, May 1, 2008; 133(5): 1101 - 1106.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Abel and H. Vliagoftis
Mast Cell-Fibroblast Interactions Induce Matrix Metalloproteinase-9 Release from Fibroblasts: Role for IgE-Mediated Mast Cell Activation
J. Immunol., March 1, 2008; 180(5): 3543 - 3550.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-P. Han, C. Yan, L. Zhou, L. Qin, and H. Tsukamoto
A Matrix Metalloproteinase-9 Activation Cascade by Hepatic Stellate Cells in Trans-differentiation in the Three-dimensional Extracellular Matrix
J. Biol. Chem., April 27, 2007; 282(17): 12928 - 12939.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. M. Kelly, R. Leigh, S. E. Gilpin, E. Cheng, G. E. M. Martin, K. Radford, G. Cox, and J. Gauldie
Cell-specific Gene Expression in Patients with Usual Interstitial Pneumonia
Am. J. Respir. Crit. Care Med., September 1, 2006; 174(5): 557 - 565.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Shukla, T. F. Barrett, K. I. Nakayama, K. Nakayama, B. T. Mossman, and K. M. Lounsbury
Transcriptional up-regulation of MMP12 and MMP13 by asbestos occurs via a PKC{delta}-dependent pathway in murine lung
FASEB J, May 1, 2006; 20(7): 997 - 999.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P T G Elkington and J S Friedland
Matrix metalloproteinases in destructive pulmonary pathology
Thorax, March 1, 2006; 61(3): 259 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Fujita, Q. Ye, H. Ouchi, E. Harada, I. Inoshima, K. Kuwano, and Y. Nakanishi
Doxycycline Attenuated Pulmonary Fibrosis Induced by Bleomycin in Mice
Antimicrob. Agents Chemother., February 1, 2006; 50(2): 739 - 743.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Matsuyama, M. Watanabe, Y. Shirahama, R. Hirano, H. Mitsuyama, I. Higashimoto, M. Osame, and K. Arimura
Suppression of Discoidin Domain Receptor 1 by RNA Interference Attenuates Lung Inflammation
J. Immunol., February 1, 2006; 176(3): 1928 - 1936.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
A. Pardo and M. Selman
Matrix metalloproteases in aberrant fibrotic tissue remodeling.
Proceedings of the ATS, January 1, 2006; 3(4): 383 - 388.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. Matsuyama, H. Mitsuyama, M. Watanabe, Y. Shirahama, I. Higashimoto, M. Osame, and K. Arimura
Involvement of Discoidin Domain Receptor 1 in the Deterioration of Pulmonary Sarcoidosis
Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 565 - 573.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Matsuyama, M. Watanabe, Y. Shirahama, K.-i. Oonakahara, I. Higashimoto, T. Yoshimura, M. Osame, and K. Arimura
Activation of Discoidin Domain Receptor 1 on CD14-Positive Bronchoalveolar Lavage Fluid Cells Induces Chemokine Production in Idiopathic Pulmonary Fibrosis
J. Immunol., May 15, 2005; 174(10): 6490 - 6498.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. E. Starr, T. Dan, K. Minhas, P. E. Shewen, and B. L. Coomber
Potential Involvement of Gelatinases and Their Inhibitors in Mannheimia haemolytica Pneumonia in Cattle
Infect. Immun., August 1, 2004; 72(8): 4393 - 4400.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
I. I. Ekekezie, D. W. Thibeault, S. D. Simon, M. Norberg, J. D. Merrill, R. A. Ballard, P. L. Ballard, and W. E. Truog
Low Levels of Tissue Inhibitors of Metalloproteinases With a High Matrix Metalloproteinase-9/Tissue Inhibitor of Metalloproteinase-1 Ratio Are Present in Tracheal Aspirate Fluids of Infants Who Develop Chronic Lung Disease
Pediatrics, June 1, 2004; 113(6): 1709 - 1714.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. G. Vazquez de Lara, T. M. Umstead, S. E. Davis, and D. S. Phelps
Surfactant protein A increases matrix metalloproteinase-9 production by THP-1 cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L899 - L906.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. Betsuyaku, K. Kadomatsu, G. L. Griffin, T. Muramatsu, and R. M. Senior
Increased Basigin in Bleomycin-Induced Lung Injury
Am. J. Respir. Cell Mol. Biol., May 1, 2003; 28(5): 600 - 606.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. J. Atkinson and R. M. Senior
Matrix Metalloproteinase-9 in Lung Remodeling
Am. J. Respir. Cell Mol. Biol., January 1, 2003; 28(1): 12 - 24.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H.J. Bunn, G. Woltmann, and J. Grigg
Applicability of laser scanning cytometry to study paediatric alveolar macrophages
Eur. Respir. J., December 1, 2002; 20(6): 1437 - 1443.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M.T. Henry, K. McMahon, A.J. Mackarel, K. Prikk, T. Sorsa, P. Maisi, R. Sepper, M.X. FitzGerald, and C.M. O'Connor
Matrix metalloproteinases and tissue inhibitor of metalloproteinase-1 in sarcoidosis and IPF
Eur. Respir. J., November 1, 2002; 20(5): 1220 - 1227.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Eberhardt, E.-S. Akool, J. Rebhan, S. Frank, K.-F. Beck, R. Franzen, F. M. A. Hamada, and J. Pfeilschifter
Inhibition of Cytokine-induced Matrix Metalloproteinase 9 Expression by Peroxisome Proliferator-activated Receptor alpha Agonists Is Indirect and Due to a NO-mediated Reduction of mRNA Stability
J. Biol. Chem., August 30, 2002; 277(36): 33518 - 33528.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
W. Eberhardt, M. Schulze, C. Engels, E. Klasmeier, and J. Pfeilschifter
Glucocorticoid-Mediated Suppression of Cytokine-Induced Matrix Metalloproteinase-9 Expression in Rat Mesangial Cells: Involvement of Nuclear Factor-{kappa}B and Ets Transcription Factors
Mol. Endocrinol., August 1, 2002; 16(8): 1752 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. G. Crystal, P. B. Bitterman, B. Mossman, M. I. Schwarz, D. Sheppard, L. Almasy, H. A. Chapman, S. L. Friedman, T. E. King Jr., L. A. Leinwand, et al.
Future Research Directions in Idiopathic Pulmonary Fibrosis: Summary of a National Heart, Lung, and Blood Institute Working Group
Am. J. Respir. Crit. Care Med., July 15, 2002; 166(2): 236 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
L. Zheng, W.K. Lam, G.L. Tipoe, I.H. Shum, C. Yan, R. Leung, J. Sun, G.C. Ooi, and K.W. Tsang
Overexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo
Eur. Respir. J., July 1, 2002; 20(1): 170 - 176.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. K. Winkler and J. L. Fowlkes
Metalloproteinase and growth factor interactions: do they play a role in pulmonary fibrosis?
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L1 - L11.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
K. H. Choi, H. B. Lee, M. Y. Jeong, Y. K. Rhee, M. J. Chung, Y. G. Kwak, and Y. C. Lee
The Role of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Cryptogenic Organizing Pneumonia*
Chest, May 1, 2002; 121(5): 1478 - 1485.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Zuo, N. Kaminski, E. Eugui, J. Allard, Z. Yakhini, A. Ben-Dor, L. Lollini, D. Morris, Y. Kim, B. DeLustro, et al.
Gene expression analysis reveals matrilysin as a key regulator of pulmonary fibrosis in mice and humans
PNAS, April 30, 2002; 99(9): 6292 - 6297.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
K. Cederqvist, T. Sorsa, T. Tervahartiala, P. Maisi, K. Reunanen, P. Lassus, and S. Andersson
Matrix Metalloproteinases-2, -8, and -9 and TIMP-2 in Tracheal Aspirates From Preterm Infants With Respiratory Distress
Pediatrics, September 1, 2001; 108(3): 686 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Ramos, M. Montaño, J. García-Alvarez, V. Ruiz, B. D. Uhal, M. Selman, and A. Pardo
Fibroblasts from Idiopathic Pulmonary Fibrosis and Normal Lungs Differ in Growth Rate, Apoptosis, and Tissue Inhibitor of Metalloproteinases Expression
Am. J. Respir. Cell Mol. Biol., May 1, 2001; 24(5): 591 - 598.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Araya, M. Maruyama, K. Sassa, T. Fujita, R. Hayashi, S. Matsui, T. Kashii, N. Yamashita, E. Sugiyama, and M. Kobayashi
Ionizing radiation enhances matrix metalloproteinase-2 production in human lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2001; 280(1): L30 - L38.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Betsuyaku, Y. Fukuda, W. C. Parks, J. M. Shipley, and R. M. Senior
Gelatinase B Is Required for Alveolar Bronchiolization after Intratracheal Bleomycin
Am. J. Pathol., August 1, 2000; 157(2): 525 - 535.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W.-Y. Su, R. H. Jaskot, J. Richards, S. R. Abramson, J. F. Woessner Jr., W.-H. Yu, and K. L. Dreher
Induction of pulmonary matrilysin expression by combustion and ambient air particles
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L152 - L160.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
L. Segura-Valdez, A. Pardo, M. Gaxiola, B. D. Uhal, C. Becerril, and M. Selman
Upregulation of Gelatinases A and B, Collagenases 1 and 2, and Increased Parenchymal Cell Death in COPD
Chest, March 1, 2000; 117(3): 684 - 694.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society. 		  ATS Best of the Web