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Am. J. Respir. Cell Mol. Biol., Volume 20, Number 6, June, 1999 1319-1325

Expression of Nerve Growth Factor-Induced Clone B Subfamily and Pro-opiomelanocortin Gene in Lung Cancer Cell Lines

Yutaka Ueda, Shuji Bandoh, Jiro Fujita, Makoto Sato, Yasufumi Yamaji, and Jiro Takahara

First Department of Internal Medicine, Kagawa Medical University; and Department of Internal Medicine, Mitoyo General Hospital, Kagawa, Japan

Nerve growth factor-induced clone B (NGFI-B), Nur-related factor 1, and neuron-derived orphan receptor-1 have structural features of ligand-activated transcriptional regulators and constitute the NGFI-B subfamily within the nuclear receptor superfamily. The NGFI-B subfamily is highly expressed in neuroendocrine organs and regulates the pro-opiomelanocortin (POMC) gene. Small-cell lung cancer (SCLC) is considered to be a neuroendocrine tumor that produces large numbers of polypeptide hormones. In this study we measured the NGFI-B subfamily and POMC messenger RNA (mRNA) levels in various lung-cancer cell lines by means of the quantitative reverse transcription-polymerase chain reaction, and evaluated the correlations between expression of these genes and polypeptide hormone productions. We also examined the effect of antisense oligonucleotide to NGFI-B mRNA on the expression of POMC mRNA. The NGFI-B subfamily and POMC mRNAs were highly expressed in SCLC cell lines. In addition, there were strong correlations between the NGFI-B, POMC genes, and the adrenocorticotropin hormone (ACTH) level. Further, the antisense oligonucleotide significantly suppressed POMC gene expression. We conclude that the NGFI-B subfamily was a significant molecule in SCLC and that the NGFI-B was a positive transcriptional factor for ACTH production.




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Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society. 		  2009 ATS Conference