help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohman, T.
Right arrow Articles by Jackson, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohman, T.
Right arrow Articles by Jackson, R. M.

Am. J. Respir. Cell Mol. Biol., Volume 21, Number 1, July, 1999 119-127

Hypoxic Modulation of Manganese Superoxide Dismutase Promoter Activity and Gene Expression in Lung Epithelial Cells

Tauni Ohman, Gregory Parish, and Robert M. Jackson

Birmingham Department of Veterans Affairs Medical Center; and University of Alabama at Birmingham, Birmingham, Alabama

We investigated the effects of hypoxia (< 2.5% O2) on rat manganese superoxide dismutase (MnSOD) gene promoter-luciferase reporter constructs in transiently transfected lung epithelial cells (A549, L2, and E1A-T2) and fibroblasts (R9Ab). We cloned MnSOD promoter-luciferase reporter constructs (numbers refer to length in base pairs [bp] in the 5' direction from the transcription initiation site): 2,505, 1,064, 507, 405, and 289 into pGL2-Basic, a promoterless, firefly luciferase vector. Lung cells were transfected with MnSOD promoter-reporter constructs with or without thymidine kinase-driven Renilla luciferase (pRL-TK), and were exposed to air/5% CO2 or hypoxia (2.5% O2/5% CO2/balance N2) for 24 h. Hypoxia caused a significant (by two-way analysis of variance) consistent increase in luciferase in the A549 cell (human lung carcinoma) line. Greatest expression (> 3-fold increase) in hypoxia was associated with the 2,505-bp MnSOD promoter (normalized to cellular protein). Azide (10 µM) did not increase expression of the MnSOD reporter constructs. The 289-bp promoter was sufficient to express the reporter in air and to increase its expression in hypoxia. Promoter activity of the rat MnSOD 5' region, assessed by luciferase reporter constructs in A549 cells, increased in hypoxia. The increase was exclusive to A549 cells and did not occur in other cells.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
V. L. Kinnula and J. D. Crapo
Superoxide Dismutases in the Lung and Human Lung Diseases
Am. J. Respir. Crit. Care Med., June 15, 2003; 167(12): 1600 - 1619.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. F. Mueller, C. Ma, J. A. Lederer, and D. L. Perkins
Differentiation of stress, metabolism, communication, and defense responses following transplantation
J. Leukoc. Biol., March 1, 2003; 73(3): 379 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Li and R. M. Jackson
Reactive species mechanisms of cellular hypoxia-reoxygenation injury
Am J Physiol Cell Physiol, February 1, 2002; 282(2): C227 - C241.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society. 		  2009/2010 ATS Fellows Career Development Awards